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(newest | oldest) View (newer 50 | older 50) (20 | 50 | 100 | 250 | 500)- 13:10, 13 February 2024 Bart talk contribs created page Competent Yeast Cells Without Synchronization (Generated protocol without synchronization) Tag: Visual edit
- 14:38, 24 July 2023 Bart talk contribs created page Malt extract mating medium (Created page with "Mating medium based on malt extract (ME). {| class="wikitable" | colspan="2" rowspan="1" |'''ME: Malt extract (per liter)''' |- | colspan="1" rowspan="1" | '''Per lit...") Tag: Visual edit
- 14:34, 24 July 2023 Bart talk contribs created page Mating medium (Created page) Tag: Visual edit
- 10:50, 11 May 2023 Bart talk contribs created page Yeast Colony PCR FastGene Nippon (Created page with "Category:Protocols ==Colony PCR of yeast cells using TopTaq== Because QIAGEN for some unknown reason (probably money) discontinued the TopTaq kits, we will finish the cu...") Tag: Visual edit
- 14:41, 13 January 2023 Bart talk contribs created page Drop out medium (Created page with "Category:Media Category:Media ==Drop out medium (DO-xxx)== To test for deficiency of strains, use a defined minimal medium (we use [[PMG - Pombe Minimal Glutamate|PMG]...") Tag: Visual edit
- 12:40, 7 December 2022 User account Andrea talk contribs was created by Admin talk contribs and password was sent by email
- 15:06, 25 November 2022 Admin talk contribs created page Selection Media (SM) (Created page with "Selection Media (SM) Category:Media") Tag: Visual edit
- 15:03, 25 November 2022 User account Russell talk contribs was created by Admin talk contribs and password was sent by email
- 11:42, 14 November 2022 User account Mei talk contribs was created by Admin talk contribs and password was sent by email
- 16:18, 13 October 2022 Bart talk contribs created page Contaminations and what to do about them (Generated page) Tag: Visual edit
- 15:24, 9 May 2022 Bart talk contribs created page SpEDIT CRISPR/Cas9 method Full protocol (Created page with "dfd")
- 14:47, 2 May 2022 Bart talk contribs created page Fluorescent protein fixation for FACS (Created page with "==Fixation Technique== '''!!! For this protocol, work in the hood !!!''' [http://mcb.berkeley.edu/labs/koshland/Protocols/MICROSCOPY/gfpfix.html Based on this protocol] #Spin...") Tag: Visual edit
- 13:02, 8 March 2022 Bart talk contribs created page Cellophane for mycelial harvesting (Created page with "We use cellophane membrane on top of an agar layer to grow mycelium. The nutrients are able to diffuse through the membrane, but the fungal mycelium is not able to grow into t...") Tag: Visual edit
- 15:22, 3 March 2022 Cristina talk contribs created page Protoplasts (Created a new protocol for protoplast generation) Tag: Visual edit
- 14:40, 7 December 2021 Bart talk contribs created page Supplements (Created page with "Standard concentrations for supplements for YES, EMM etc.<BR> When 10% of normal is required, you have to reduce this amount to 10%.<BR> The supplements are stored as stock so...") Tag: Visual edit: Switched
- 14:57, 2 August 2021 Bart talk contribs created page Benito (Redirected page to Selection medium Pombe Benito et al) Tags: New redirect Visual edit
- 10:29, 11 May 2021 User account Murphy talk contribs was created by Admin talk contribs and password was sent by email
- 16:24, 27 April 2021 User account Dmytro talk contribs was created by Admin talk contribs and password was sent by email
- 12:14, 9 March 2021 Bart talk contribs created page DNA Extraction S. commune QIAgen MagAttract (Created page with "#Spin down the protoplasts and mycelium rests at 5000 rcf for 5 minutes #Transfer 180µl of the '''supernatant''' to a fresh 2ml sample tube #Continue with step 5. on page 26...") Tag: Visual edit
- 12:31, 22 February 2021 User account Chaitra talk contribs was created by Admin talk contribs and password was sent by email
- 17:08, 4 February 2021 Bart talk contribs created page DNA Extraction S. commune Phenol Chlorophorm PEG (Created page with "DNA extraction for ''Schizophyllum commune'' as described in van Peer et al. 2009 <ref name="peer">[https://doi.org/10.1128/AEM.02162-08 Peer, Arend F. van, Charissa de Bekker...") Tag: Visual edit
- 13:56, 4 February 2021 Admin talk contribs created page Grow mycelia for DNA extractions (Created page with "For mycelia, DNA extractions are more difficult than for yeast cells, because we need to: *grow the mycelium *harvest the mycelium *freeze dry the mycelium *break the myceliu...") Tag: Visual edit
- 14:37, 29 January 2021 Admin talk contribs created page TissueLyser Bead Cleanup (Created page with "In general, collect beads in 50mL tube with demi-water and keep them there until cleaning is needed. Washing: *Remove water *Add fresh 25 mL demi-water *Vortex for several se...") Tag: Visual edit
- 12:18, 20 January 2021 Admin talk contribs created page Schizophyllum mating type test crosses (Created page with "Test for mating type in ''Schizophyllum commune'' This is based on the paper by Papazian (Papazian 1950). Mating type in ''Schizophyllum'' is defined by two unlinked loci (mat...")
- 18:02, 4 January 2021 Bart talk contribs created page Transformations of Yeast (Created page with "There are a variety of methods to transform yeast. === Chemical transformations is '''used most''' in the lab === * First make Synchronized Competent Yeast Cells (Takes...") Tag: Visual edit
- 18:18, 3 January 2021 Bart talk contribs moved page Yeast Colony PCR to Yeast Colony PCR TopTaq
- 17:42, 3 January 2021 Bart talk contribs created page Colony PCR (Created page with "Colony PCR is a simple method to amplify DNA in which cells are directly used as template. This method can be used both for: *Yeast grown on a plate *a...") Tag: Visual edit
- 17:37, 3 January 2021 Bart talk contribs moved page OLD Yeast Electoporation Transformation OLD to Yeast Electoporation Transformation OLD!!
- 17:17, 3 January 2021 Bart talk contribs created page Miniprep kit Macherey-Nagel (Created page with "== Mini-prep of plasmids using kit (''E. coli'') == Using kit from Macherey-Nagel * Grow cells over night in 5ml liquid LB medium * Spin down cells from 2 ml in 2ml tube at 1...")
- 17:15, 3 January 2021 Bart talk contribs created page Miniprep (Created page with "Minipreps are methods to extract plasmid DNA molecules from ''E. coli'' or ''Yeast cells''. In our lab we use a variety of methods. # Easiest is to use a Miniprep kit Mach...")
- 17:06, 3 January 2021 Bart talk contribs created page Sterilize (Created page with "Sterilization can be performed by: * Autoclave when the compound/sample/tube etc can be heated to above 121C * Filter sterilization fo...")
- 16:59, 3 January 2021 Bart talk contribs moved page Big Dye Setup to Sanger Sequencing - Big Dye Setup (Logical name)
- 16:56, 3 January 2021 Bart talk contribs created page User:Bart (Created page with "I'm PI of the PombEvolution group at LMU Munich. My research focusses on the evolution of divergence between reproducing organisms. Mostly on the evolution of mating types an...")
- 12:28, 18 August 2020 PilarH talk contribs uploaded File:---Category-Protocols-Protocols--.png (Making diploids)
- 13:16, 14 August 2020 User account PilarH talk contribs was created by Admin talk contribs
- 16:28, 14 July 2020 User account Cristina talk contribs was created by Admin talk contribs and password was sent by email
- 15:08, 17 June 2020 Bart talk contribs moved page QIAGEN Genomic-Tip to DNA extraction QIAGEN Genomic-Tip S. pombe
- 12:41, 20 April 2020 Bart talk contribs uploaded File:Fig7c clipboard.png (Figure 7c from Rodríguez-López et al 2017. https://wellcomeopenresearch.org/articles/1-19/v3)
- 11:37, 6 April 2020 Bart talk contribs moved page Pombe Colony PCR to Yeast Colony PCR
- 12:55, 2 March 2020 User account Michaela talk contribs was created by Admin talk contribs
- 11:56, 31 January 2019 Admin talk contribs deleted page File:Neubauer improved.jpg (Deleted old revision 20190131095627!Neubauer_improved.jpg)
- 11:56, 31 January 2019 Admin talk contribs uploaded a new version of File:Neubauer improved.jpg
- 11:53, 31 January 2019 Admin talk contribs uploaded File:Burker turk.jpg
- 11:46, 31 January 2019 Admin talk contribs uploaded File:Neubauer improved.jpg
- 11:34, 31 January 2019 Admin talk contribs uploaded File:Haemocytometer.png (Schematic view of a haemocytometer. Image from http://www.ruf.rice.edu/~bioslabs/methods/microscopy/)
- 13:52, 9 January 2019 User account Sebastian talk contribs was created by Admin talk contribs
- 15:15, 7 November 2018 User account Pilar talk contribs was created by Admin talk contribs
- 15:15, 7 November 2018 User account Linda talk contribs was created by Admin talk contribs
- 15:14, 7 November 2018 User account Mohammed talk contribs was created by Admin talk contribs and password was sent by email
- 13:04, 30 October 2018 Bart talk contribs moved page Yeast Electoporation Transformation Old to OLD Yeast Electoporation Transformation OLD