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Category:Protocols

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Protocols

Pages in category "Protocols"

The following 43 pages are in this category, out of 43 total.

A

  • Autoclaving

C

  • Calcofluor white fungal cell wall staining
  • Cell viability assay AO and PI
  • Cellophane for mycelial harvesting
  • Cheap miniprep
  • Colony PCR
  • Creating diploids
  • CRISPR Cas9 Full protocol
  • Crossing two strains

D

  • Determine concentration of yeast
  • DNA concentration with the NanoDrop
  • DNA extraction QIAGEN Genomic-Tip S. pombe
  • DNA Extraction QIAGEN Kit

E

  • E coli Chemical Transformations
  • E coli colony PCR
  • ExoSap PCR cleanup

F

  • Filter sterilize

G

  • Glycerol stock
  • Golden Gate

I

  • Iodine staining

K

  • Kill all but spores

M

  • Mating type determination
  • Miniprep
  • Miniprep kit Macherey-Nagel

P

  • PCR for mat1
  • Phusion colony PCR

R

  • Rubidiumchlorid competent E.coli cells

S

  • Sanger Sequencing - Big Dye Setup
  • Schizophyllum mating type test crosses
  • Single cell colony
  • SpEDIT CRISPR/Cas9 method Full protocol
  • Stitch PCR
  • Storage of Media
  • Storage of Strains
  • Synchronized Competent Yeast Cells

T

  • Test crosses
  • TissueLyser Bead Cleanup
  • Transformations of Yeast

Y

  • Yeast Chemical Transformations
  • Yeast Colony PCR TopTaq
  • Yeast Colony Transformations
  • Yeast Electoporation Transformation
  • Yeast Electoporation Transformation OLD!!
Retrieved from "https://www.pombevolution.eu/labwiki/index.php?title=Category:Protocols&oldid=18"

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  • This page was last edited on 22 September 2018, at 18:16.
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