Buffer QF
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Jump to navigationJump to searchBuffer QF (elution buffer) used for the QIAGEN Genomic-Tip DNA extraction protocol.
The buffer is used to elute the DNA from the column.
Buffer QF
- 1.25 M NaCl
- 50 mM Tris·Cl, pH 8.5
- 15% isopropanol
Buffer QF can be stored at RT.
To make QF:
- Dissolve in 200 ml distilled water:
- 18.26 g NaCl
- 1.52 g Tris base
- Adjust the pH to 8.5 with HCl
- Add 37.5 ml pure isopropanol
- Adjust the volume to 250 ml with distilled water.