Transformations of Yeast

From PombEvolution
Jump to navigationJump to search

There are a variety of methods to transform yeast.

Chemical transformations is used most in the lab

This method is most convenient and rather successful in our hands.

Alternatively use electroporation

In theory, this should yield higher transformation efficiency, however, this is in our experience not worth the effort of setting up the equipment.

Chemical transformations from yeast colonies

This method is convenient as it takes little prep time to generate competent cells, but has very low transformation efficiency.