TissueLyser Bead Cleanup

In general, collect beads in 50mL tube with demi-water and keep them there until cleaning is needed.

Washing:

• Remove water
• Add fresh 25 mL demi-water
• Vortex for several seconds
• Pour off water (collect water for proper disposal; assume liquid contains GMO)
• If beads are not clean, wipe beads clean with tissue paper
• Add 20mL 0.1M NaOH in MilliQ (for RNAse free beads, add [DEPC])
• Incubate overnight
• Pour out solution
• Wash beads with MilliQ (DEPC treated MilliQ for RNAse free) and pour out solution after each step.
• Transfer beads to sterile Petri dish and let dry in incubator (ON at 32°C or 37°C should be enough time)
• Store in clean tube