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• Malt extract mating medium
  | colspan="2" rowspan="1" |'''ME: Malt extract (per liter)''' | colspan="1" rowspan="1" |  '''Per liter'''
  755 bytes (103 words) - 14:41, 24 July 2023
• Tris
  ==For 1 L== * Dissolve 121.1 g Tris base in 800 mL of ddH₂O
  255 bytes (45 words) - 13:10, 30 October 2018
• Vitamin Stock x1000
  |0.1 g || pantothenic acid || 81.2 mM || pantothenic acid |1 g || nicotinic acid || 81.2 mM || nicotinic acid
  632 bytes (83 words) - 11:36, 26 April 2021
• Schizophyllum strains
  | Vreia de Jales, Portugal || 181103 || 181207 || Vreia-1 || || || || || || || || 41.433936, -7.596481 | Kothe || || 180815 || 1-6 || B1 || || 9 || 22 || 3 || 7 || ||
  3 KB (157 words) - 12:21, 6 December 2018
• Modified Schizophyllum Minimal Medium
  !Per 1 liter | K₂HPO₄. 3 H₂O || 1.28 g || 2.56 g
  999 bytes (136 words) - 11:57, 18 August 2020
• Schizophyllum Minimal Medium
  !Per 1 liter | K₂HPO₄. 3 H₂O || 1.28 g
  759 bytes (102 words) - 13:44, 20 November 2018
• Nourseothricin
  * dissolving 1 g [https://www.jenabioscience.com Nourseothricin] Nourseothricin-salt in 10 Add 1:1000 (i.e. 25µl/plate) to solid medium of ~50-60°C to obtain a final conc
  677 bytes (101 words) - 16:50, 23 November 2018
• Kanamycin stock
  *Stock may be kept at -20°C for 1 year. '''Usage:''' dilute 1:1000 to final concentration of 50µl/ml (25µl for 9cm plate)
  464 bytes (70 words) - 19:30, 22 January 2019
• Hygromycin
  * dissolving 1 g hygromycin in 10ml H₂O and filter sterilize. Add 1:1000 (i.e. 25µl/plate) to solid medium of ~50-60°C to obtain a final conc
  853 bytes (136 words) - 16:59, 23 November 2018
• SOC
  |MgCl₂ 1M (final concentration 10 mM] || 1 ml *add 1 ml of MgCl₂ from the 1M Stock solution ( fridge 3).
  1 KB (155 words) - 17:02, 27 January 2019
• Tris-EDTA buffer
  * 1 mM [[EDTA]] TE buffer is also called as T₁₀E₁ Buffer, and read as "T ten E one buffer".
  921 bytes (155 words) - 13:10, 30 October 2018
• PLATE
  |1 ml || [[Lithium Acetate 1M]] || 100 mM |0.1 ml || [[Tris|Tris-HCl 1 M]] || 10 mM
  574 bytes (75 words) - 15:11, 7 November 2018
• Y1
  *1 M sorbitol *Add 1 ml of β-mercaptoethanol (14.3 M).
  800 bytes (120 words) - 19:15, 22 January 2019
• Stitch PCR
  == PCR 1 == ...on'' PCR, like normal, but using a 1:10 dilution of the products of '''PCR 1''' as template.
  2 KB (321 words) - 16:37, 4 October 2018
• Sorbitol 1M
  To make 1 liter of 1M sorbitol<br> *Fill up to 1 liter.
  571 bytes (78 words) - 12:58, 23 September 2018
• Sanger Sequencing - Big Dye Setup
  |1 μl || [[Cheap miniprep | Miniprep]] or clean PCR product |1 μl || Primer (e.g. M13F) (10 μM)
  1 KB (217 words) - 16:59, 7 June 2023
• G418 - Geneticin
  Use the stock solution (fridge 1) at 1 in 500 dilution (i.e. 50 μl/9cm plate or 20 μl/4.5cm plate).
  676 bytes (107 words) - 10:56, 20 January 2021
• Phusion colony PCR
  |dNTPs (10 mM each)|| 1 μl |Primer Fwd (10 μM) || 1 μl
  1 KB (195 words) - 21:42, 3 January 2021
• Golden Gate
  * Calculate the dilution to obtain 13.3 ng/μl per 1-kilobase DNA (for a plasmid: insert length + 3.5kb) * mix 1 μl of each plasmid
  1 KB (156 words) - 14:56, 10 December 2018
• Mineral Stock x10.000
  !Amount per 1 liter |1 g || Potassium iodide || 6.02 mM || KI
  916 bytes (124 words) - 12:30, 23 September 2018

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