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Solution used for Yeast Colony Transformations

Medium is similar to the final mixture in Yeast Chemical Transformations.

Amount per 10 mL Final Concentration
7 ml 50% Polyethylene glycol (PEG)‐4000 35%
1 ml Lithium Acetate 1M 100 mM
0.1 ml Tris-HCl 1 M 10 mM
20 µl 0.5 M EDTA, pH 8.0 1mM
add to get a final total volume of 10 ml H2O -

Filter sterilize and store in fridge