Difference between revisions of "PMG - Pombe Minimal Glutamate"
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== Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | == Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | ||
| − | Modification of [[EMM_-_Edinburgh_Minimal_Medium|EMM]] in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on [[EMM]] (especially G418). On this medium strains do [[Crossing two strains|cross]] so the medium can also be used to [[Mating type determination|test for mating types]]. However, it is much richer than the crossing mediums [[EMM-lowN]] or [[EMM-N_-_Minimal_Mating_Medium|EMM-N]], so mating takes more time (about 3 days).BR> | + | Modification of [[EMM_-_Edinburgh_Minimal_Medium|EMM]] in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on [[EMM]] (especially G418). On this medium strains do [[Crossing two strains|cross]] so the medium can also be used to [[Mating type determination|test for mating types]]. However, it is much richer than the crossing mediums [[EMM-lowN]] or [[EMM-N_-_Minimal_Mating_Medium|EMM-N]], so mating takes more time (about 3 days).<BR> |
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Revision as of 13:50, 19 March 2020
Edinburgh Minimal Medium Glutamate (EMMG, PMG)
Modification of EMM in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on EMM (especially G418). On this medium strains do cross so the medium can also be used to test for mating types. However, it is much richer than the crossing mediums EMM-lowN or EMM-N, so mating takes more time (about 3 days).
| Ingredient | Amount per liter |
|---|---|
| Potassium Hydrogen Phthallate | 3.0 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) |
| L-Glutamic acid monosodium salt hydrate (glutamate) | 5.0 g |
| D-Glucose | 20 g (2%) |
| Salts Stock x50 | 20 ml |
| Vitamin Stock x1000 | 1.0 ml |
| Mineral Stock x10.000 | 0.1 ml |
Add supplements as required (225mg/L for most, 100mg/L for Adenine).
For solid medium add 2% Agar-agar (20g/L)
