Difference between revisions of "PMG - Pombe Minimal Glutamate"
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[[Category:Media]] | [[Category:Media]] | ||
== Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | == Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | ||
| − | Modification of [[EMM_-_Edinburgh_Minimal_Medium|EMM]] in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on [[EMM]] (especially G418). On this medium strains do [[Crossing two strains|cross]] so the medium can also be used to [[Mating type determination|test for mating types]]. However, it is much richer than the crossing mediums [[EMM-lowN]] or [[EMM-N_-_Minimal_Mating_Medium|EMM-N]], so mating takes more time (about 3 days).<BR> | + | Modification of [[EMM_-_Edinburgh_Minimal_Medium|EMM]] in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on [[EMM]] (especially G418). On this medium strains do [[Crossing two strains|cross]] so the medium can also be used to [[Mating type determination|test for mating types]]. However, it is much richer than the crossing mediums [[EMM-lowN]] or [[EMM-N_-_Minimal_Mating_Medium|EMM-N]], so mating takes more time (about 3 days). |
| + | |||
| + | If available, use the [https://www.mpbio.com/eu/114110712-emm-no-nitrogen-no-nh-4-cl-g premixed bottle] which contains all ingredients, except nitrogen source (and supplements and agar if needed), which have to be added separately.<BR> | ||
{| class="wikitable" | {| class="wikitable" | ||
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|Na<sub>2</sub>HPO<sub>4</sub>.2H<sub>2</sub>O (or anhydrous)||2.76 g (2.2 g) | |Na<sub>2</sub>HPO<sub>4</sub>.2H<sub>2</sub>O (or anhydrous)||2.76 g (2.2 g) | ||
|- | |- | ||
| − | |L-Glutamic acid monosodium salt hydrate ( | + | |L-Glutamic acid monosodium salt hydrate |
| + | (or L-Glutamic acid) | ||
| + | | 5.0 g (3.75 g) | ||
|- | |- | ||
|D-Glucose || 20 g (=2% final concentration) | |D-Glucose || 20 g (=2% final concentration) | ||
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|[[Mineral Stock x10.000]] ||0.1 ml | |[[Mineral Stock x10.000]] ||0.1 ml | ||
|} | |} | ||
| + | |||
| + | When using L-Glutamic acid, set to pH 5.8 using NaOH 10N | ||
Add supplements as required (225mg/L for most, 100mg/L for Adenine).<BR> | Add supplements as required (225mg/L for most, 100mg/L for Adenine).<BR> | ||
For solid medium add 2% Agar-agar (20g/L) | For solid medium add 2% Agar-agar (20g/L) | ||
Latest revision as of 14:32, 13 January 2023
Edinburgh Minimal Medium Glutamate (EMMG, PMG)
Modification of EMM in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on EMM (especially G418). On this medium strains do cross so the medium can also be used to test for mating types. However, it is much richer than the crossing mediums EMM-lowN or EMM-N, so mating takes more time (about 3 days).
If available, use the premixed bottle which contains all ingredients, except nitrogen source (and supplements and agar if needed), which have to be added separately.
| Ingredient | Amount per liter |
|---|---|
| Potassium Hydrogen Phthallate | 3.0 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) |
| L-Glutamic acid monosodium salt hydrate
(or L-Glutamic acid) |
5.0 g (3.75 g) |
| D-Glucose | 20 g (=2% final concentration) |
| Salts Stock x50 | 20 ml |
| Vitamin Stock x1000 | 1.0 ml |
| Mineral Stock x10.000 | 0.1 ml |
When using L-Glutamic acid, set to pH 5.8 using NaOH 10N
Add supplements as required (225mg/L for most, 100mg/L for Adenine).
For solid medium add 2% Agar-agar (20g/L)
