Storage of Strains
From PombEvolution
Jump to navigationJump to searchThe printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Long term cryopreservation
Yeast and E. coli strains are most safely stored by cryopreservation at -80°C in YES + 15% Glycerol.
Medium time as spores
Spore are very stable and can sustain storage at 4°C for prolonged time, up to multiple months.
- Take the formed spores.
- Resuspend them in sterile water.
- Place at 4°C.
- Haploid and diploid cells will mostly not survive this treatment for more than a few weeks.
