Storage of Strains
From PombEvolution
Jump to navigationJump to searchLong term cryopreservation
Yeast and E. coli strains are most safely stored by cryopreservation at -80°C in YES + 15% Glycerol.
- Grow strains while shaking in ~2-5ml YES medium till late exponential phase
- Spin down the cells
- Resuspend cells in YES + 15% Glycerol
- Immidately place the appropriate box in the -80°C.
- Document your strains in the strains catalog and in the -80°C document in the lab Dropbox
Medium time as spores
Spore are very stable and can sustain storage at 4°C for prolonged time, up to multiple months.
- Take the formed spores.
- Resuspend them in sterile water.
- Place at 4°C.
- Haploid and diploid cells will mostly not survive this treatment for more than a few weeks.
