Difference between revisions of "Storage of Strains"
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== Long term cryopreservation == | == Long term cryopreservation == | ||
| − | Yeast strains are most safely stored by cryopreservation at -80°C in [[YES_%2B_15%25_Glycerol| YES + 15% Glycerol]]. | + | Yeast and [[Glycerol_stock#E._coli | ''E. coli'']] strains are most safely stored by [[Glycerol_stock |cryopreservation at -80°C]] in [[YES_%2B_15%25_Glycerol| YES + 15% Glycerol]]. |
* Grow strains while shaking in ~2-5ml YES medium till late exponential phase | * Grow strains while shaking in ~2-5ml YES medium till late exponential phase | ||
Revision as of 17:10, 29 January 2019
Long term cryopreservation
Yeast and E. coli strains are most safely stored by cryopreservation at -80°C in YES + 15% Glycerol.
- Grow strains while shaking in ~2-5ml YES medium till late exponential phase
- Spin down the cells
- Resuspend cells in YES + 15% Glycerol
- Immidately place the appropriate box in the -80°C.
- Document your strains in the strains catalog and in the -80°C document in the lab Dropbox
Medium time as spores
Spore are very stable and can sustain storage at 4°C for prolonged time, up to multiple months.
- Take the formed spores.
- Resuspend them in sterile water.
- Place at 4°C.
- Haploid and diploid cells will mostly not survive this treatment for more than a few weeks.
