Phleomycin

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Antibiotic to select against yeast and fungi with

Selective antibiotic for the Sh ble gene


Make 5 ml of stock solution 20 mg/ml

  • Weigh 125 mg of Phleomycin.
  • Add 5 ml of H2O. Disolve completely.
  • Sterilize by drawing the solution through a syringe filter .

Usage

For Fission yeast and for Schizophyllum

Dilute 1:2000 to final concentration of 10 µl/ml (12µl for 9cm plate)

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