Glycerol stock

Pombe

• Grow cells overnight in 5ml liquid medium shaking.
• Spin down the cells (or leave overnight in the fridge)
• Remove supernatant
• Resuspend in YES + 15% Glycerol
• Transfer the cells to a 1.5ml (cryogen)tube
• Immediately freeze at -80°C

  register the tube in the lists

• alternatively:
  • transfer 0.5ml of liquid culture to a fresh tube
  • add 0.5ml 30% or 216 μl 50% sterile Glycerol to the tube
  • mix, store and register

Synchronized Competent Yeast Cells should be frozen in Lithium acetate + 30% Glycerol as this increases their transformation efficiency (Suga & Hatakeyama, 2005, doi:10.1002/yea.1247).

E. coli

• Grow cells overnight in LB
• Take 0.5ml
• Add 50% sterile Glycerol (in fridge 2)
• Transfer the cells to a 1.5ml (cryogen)tube
• Immediately freeze at -80°C

  register the tube in the lists