Difference between revisions of "Glycerol stock"
From PombEvolution
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* alternatively: | * alternatively: | ||
** transfer 0.5ml of liquid culture to a fresh tube | ** transfer 0.5ml of liquid culture to a fresh tube | ||
| − | ** add 0.5ml 30% sterile [[Glycerol]] to the tube | + | ** add 0.5ml 30% or 216 μl 50% sterile [[Glycerol]] to the tube |
** mix, store and '''register''' | ** mix, store and '''register''' | ||
<BR> | <BR> | ||
Revision as of 17:18, 29 January 2019
Pombe
- Grow cells overnight in 5ml liquid medium shaking.
- Spin down the cells (or leave overnight in the fridge)
- Remove supernatant
- Resuspend in YES + 15% Glycerol
- Transfer the cells to a 1.5ml (cryogen)tube
- Immediately freeze at -80°C
- register the tube in the lists
- alternatively:
- transfer 0.5ml of liquid culture to a fresh tube
- add 0.5ml 30% or 216 μl 50% sterile Glycerol to the tube
- mix, store and register
Synchronized Competent Yeast Cells should be frozen in Lithium acetate + 30% Glycerol as this increases their transformation efficiency (Suga & Hatakeyama, 2005, doi:10.1002/yea.1247).
