Difference between revisions of "Filter sterilize"
From PombEvolution
Jump to navigationJump to search (Created page with "Use filter sterilization if heat sterilization by autoclave is not possible. <BR> ===For small volumes:=== *Suck up the to be sterilized liquid into a syring...") |
|||
Line 1: | Line 1: | ||
− | Use filter sterilization if heat sterilization by [[ Autoclaving| autoclave]] is not possible. | + | Use filter sterilization if heat sterilization by [[ Autoclaving| autoclave]] is not possible. |
+ | |||
+ | Essential when making [[:Category:Stock Solutions|stock solutions]] for antibiotics and other heat sensitive compounds. | ||
+ | |||
===For small volumes:=== | ===For small volumes:=== | ||
*Suck up the to be sterilized liquid into a syringe | *Suck up the to be sterilized liquid into a syringe |
Revision as of 16:27, 31 January 2019
Use filter sterilization if heat sterilization by autoclave is not possible.
Essential when making stock solutions for antibiotics and other heat sensitive compounds.
For small volumes:
- Suck up the to be sterilized liquid into a syringe
- Attach the appropriate disk filter
- Prepare a sterile container/tube/bottle to collect the liquid in
- Press the liquid through
- Wear eye protection as the filter might shoot of if too much pressure is applied (also: don't apply too much pressure)
- The filter might get clogged when large volumes or very 'dirty' liquid is filtered. Again, be careful not too apply too much pressure when this happens. Better to change to a new filter and only then continue filtering.
- Aliquot when needed
For large volumes:
- Take a sterile bottle
- Attach a cup filter on the top of the bottle
- Connect the filter to the vacuum tap on the bench next to the waterbath
- Fill the cup with the to be filtered liquid
- Open the tap. The liquid will now be sucked through the filter. You can top up the cup during this time.
- Wait for the liquid to be completely passed through the filter and close the bottle with a sterile cap.