Storage of Strains

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Long term cryopreservation

Yeast and E. coli strains are most safely stored by cryopreservation at -80°C in YES + 15% Glycerol.

Medium time as spores

Spore are very stable and can sustain storage at 4°C for prolonged time, up to multiple months.

  • Take the formed spores.
  • Resuspend them in sterile water.
  • Place at 4°C.
  • Haploid and diploid cells will mostly not survive this treatment for more than a few weeks.

Short time on plate

Very short in liquid