Yeast Colony Transformations

Protocol based on Morita & Takegawa 2004

Fast and convenient transformation protocol for fission yeast. This method is not as efficient as the [Yeast Chemical Transformations] or the [Yeast Electoporation Transformation]. The cells are grown on a plate to form a colony that is then scraped of the medium, resuspended in transformation medium and immediately used for transformation.

1. Suspend cells of the to-be-transformed strain in sterile saline or water
2. Pipette the cells on YES in 10µl droplets
3. Grow cells for 24-48 hours on YES to form a fat colony of about 5mm
4. Prepare a tube with 50µl PLATE mixture
5. Scrape the cells of the plate with a sterile toothpick