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• Synchronized Competent Yeast Cells
  ...López et al. 2017] in which EMM-N is used to synchronize cells after which cells are stored following [https://doi.org/10.1002/yea.1247 Suga & Hatakeyama 20 These cell are used for the [[Yeast Chemical Transformations]] protocol.
  4 KB (570 words) - 14:52, 24 May 2022

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• Lithium Acetate with glycerol
  ...Synchronized Competent Yeast Cells|synchronized chemically competent yeast cells]]. Note that here we use 30% glycerol, as it increases the transformation e
  413 bytes (58 words) - 13:18, 30 October 2018
• Transformations of Yeast
  There are a variety of methods to transform yeast. * First make [[Synchronized Competent Yeast Cells]] (Takes 1 day prep + 1 day handling time)
  2 KB (245 words) - 18:06, 4 January 2021
• Yeast Chemical Transformations
  ...zation|Competent Yeast Cells]] (old version [[Synchronized Competent Yeast Cells]] has lower integration efficiency). ...aw [[Competent Yeast Cells Without Synchronization|Competent fission yeast cells]] quickly for 30 sec to RT in waterbath and put on ice
  3 KB (541 words) - 14:22, 27 March 2024
• Glycerol stock
  * Grow cells overnight in 5ml liquid YES medium shaking. * Spin down the cells (or leave overnight in the fridge)
  2 KB (252 words) - 11:48, 9 July 2020
• CRISPR Cas9 Full protocol
  === Transformation of competent ''E. coli'' cells for sgRNA === We use OneShot TOP10 Chemically competent ''E. coli'' cells (comparable to DH5α) that are to be found in the -80°C freezer (Parsch fr
  10 KB (1,704 words) - 17:11, 27 January 2023
• SpEDIT CRISPR/Cas9 method Full protocol
  ...otal 3 different plasmids (gifts from the Allshire lab) that differ in the yeast resistance marker in the backbone: === Transformation of competent ''E. coli'' cells for sgRNA ===
  11 KB (1,722 words) - 14:27, 7 December 2022