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...o a [[EMM-lowN | mating plate]] the diploids should form [[Asci | azygotic asci]].

Crosses between cells result in asci, but many of the haploid cells will be maintained and also diploids that di ...n be performed. This is fast and cheap, but will not break the wall of the asci. This is for the lab strain not required, as the spores will be released fr

* To test if the mating was successful, test for [[asci under the microscope]] and continue with [[Kill_all_but_spores#Glusulase_tr * To test if the mating was successful, test for [[asci under the microscope]] and continue with [[Kill_all_but_spores#Glusulase_tr

...-_Minimal_Mating_Medium|EMM-N]] which allows for some growth and thus more asci. Like in most fungi, low nitrogen induces mating in pombe too.<BR>Note that

# Check if no asci remain