Nourseothricin
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Revision as of 12:58, 23 September 2018 by Bart (talk | contribs) (Created page with "Nourseothricin is used a dominant resistance marker. We use it mostly in the CRISPR-Cas9 method for transformation. <BR> Make a stock solution...")
Nourseothricin is used a dominant resistance marker. We use it mostly in the CRISPR-Cas9 method for transformation.
Make a stock solution of 100mg/ml by
- dissolving 1 g Nourseothricin Nourseothricin-salt in 10ml H2O and filter sterilize.
- filter sterilize and aliquot in 1ml tubes.
Usage: add 1:1000 (i.e. 25µl/plate) to solid medium of ~50-60°C to obtain a final concentration of 100µg/ml.
