Difference between revisions of "Nourseothricin"
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| − | Nourseothricin is used a dominant resistance marker. We use it mostly in the [[CRISPR Cas9 Full protocol | CRISPR-Cas9]] method for transformation. | + | Nourseothricin is used a dominant resistance marker that is encoded by natMX. We use it mostly in the [[CRISPR Cas9 Full protocol | CRISPR-Cas9]] method for transformation. |
Make a stock solution of 100mg/ml | Make a stock solution of 100mg/ml | ||
Latest revision as of 16:50, 23 November 2018
Nourseothricin is used a dominant resistance marker that is encoded by natMX. We use it mostly in the CRISPR-Cas9 method for transformation.
Make a stock solution of 100mg/ml
- dissolving 1 g Nourseothricin Nourseothricin-salt in 10ml H2O and filter sterilize.
- filter sterilize and aliquot in 1ml tubes.
Usage
Pombe:
Add 1:1000 (i.e. 25µl/plate) to solid medium of ~50-60°C to obtain a final concentration of 100µg/ml.
Schizophyllum
Add 1:10.000 (i.e. 2.5µl/plate) to solid SMM of ~50-60°C to obtain a final concentration of 10µg/ml.
