Malt extract mating medium
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Mating medium based on malt extract (ME).
| ME: Malt extract (per liter) | |
| Per liter | Final concentration |
| 30 g Bacto-malt extract | 3% (w/v) |
| Supplements added as for YES | 225 mg/L histidine
225 mg/L uracil 225 mg/L leucine 100 mg/L adenine |
Adjust to pH 5.5 with NaOH.
Solid media is made by adding 2% w/v Difco Bacto Agar.
ME is the most common mating media, but may show batch-to-batch variation and should therefore not be used in experimental setups. For this, use EMM-lowN
