Difference between revisions of "Colony PCR"
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This method can be used both for: | This method can be used both for: | ||
| − | *[[Yeast Colony PCR|Yeast | + | *Yeast grown on a plate |
| − | *and | + | **for less complicated fragments <2.5kb use the [[Yeast Colony PCR|Yeast Colony PCR using TopTaq]] |
| + | **for difficult to amplify fragments or large fragments >2.5kb use the [[Phusion colony PCR]] | ||
| + | *and for [[E coli colony PCR|''E coli'' that was grown on plate or in liquid]] | ||
[[Category:Protocols]] | [[Category:Protocols]] | ||
Latest revision as of 17:46, 3 January 2021
Colony PCR is a simple method to amplify DNA in which cells are directly used as template.
This method can be used both for:
- Yeast grown on a plate
- for less complicated fragments <2.5kb use the Yeast Colony PCR using TopTaq
- for difficult to amplify fragments or large fragments >2.5kb use the Phusion colony PCR
- and for E coli that was grown on plate or in liquid
