Buffer G2
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Jump to navigationJump to searchBuffer G2 (digestion buffer) used for the QIAGEN Genomic-Tip DNA extraction protocol.
The buffer is used to resuspend spheroplasts and digest RNA and proteins.
Buffer G2
- 800 mM guanidine HCl
- 30 mM Tris·Cl, pH 8.0
- 30 mM EDTA, pH 8.0
- 5% Tween20
- 0.5% Triton X-100
Buffer G2 should be stored at RT or 4°C.
To make G2:
- In in 600 ml distilled water dissolve:
- 76.42 g guanidine HCl
- 11.17 g Na2EDTA·2H2O
- 3.63 g Tris base
- Add 250 ml 20% Tween-20 solution
- 50 ml 10% Triton X-100 solution
- Adjust the pH to 8.0 with NaOH
- Adjust the volume to 1 liter with distilled water
