Benomyl

Benomyl is a fungicide that depolymerises microtubules which disrupts the mitotic cell cycle. Because it is more toxic to Ascomycetes than to Basidiomycetes, it can be used to select for basidio's when isolating natural isolates.

Benomyl is of low toxicity to mammals, so work with it carefully.

Stock solution

• measure 15mg Benomyl (at RT in chemical cabinet)
• add 10ml DMSO
• Dissolve by shaking
• Filter sterilize

Usage

Add Benomyl at final concentration of 10μg/ml.

• 165 μl/ 9cm plate
• 67 μl/ small plate
• 6.7 μl/ml