YES + 15% Glycerol
From PombEvolution
Jump to navigationJump to searchThis medium is used for cryopreservation of pombe cells at -80°C.
Follow the recipe for YES without Agar but before adding ddH2O add 15% Glycerol (i.e. 150ml/liter 100% glycerol) and then top up to the final volume. Sterilize as normal by autoclaving.