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*Spread the 100 μl cells on the preheated LB+amp plates and incubate overnight at 37°C.

* Transfer 5 μl of each well to a fresh U-bottom plate with 100 μl LB+Amp and place at 37°C.

* pBN305 spedit kanMX CRISPR/Cas9 GFP GG - pLSB-kanMX [amp] --> G418 * pBN306 spedit natMX CRISPR/Cas9 GFP GG - pLSB-natMX [amp] --> nourseothricin

*Spread the 100 μl cells on the preheated LB+amp plates and incubate overnight at 37°C. ...grow each in a well of sterile PCR strips or a 96 well plate with 50 μl LB+Amp (75 μg/ml).