QBT buffer
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Jump to navigationJump to searchBuffer QBT (equilibration buffer) used for the QIAGEN Genomic-Tip DNA extraction protocol.
The buffer is used to prepare the Genomic Tip column before loading the DNA.
Buffer QBT
750 mM NaCl 50 mM MOPS, pH 7.0 15% isopropanol 0.15% Triton X-100
Buffer QBT can be stored at RT.
To make QBT:
- Dissolve in 200 ml distilled water
- 10.96 g NaCl
- 2.62 g MOPS
- Adjust the pH to 7.0 with NaOH
- Add 37.5 ml pure isopropanol
- Add 3.75 ml 10% Triton X-100 solution
- Adjust the volume to 250 ml with distilled water