Difference between revisions of "Lallzyme MMX"
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* Mix until dissolved | * Mix until dissolved | ||
* Filter sterilize when needed ('''For DNA extractions no sterilization required''') | * Filter sterilize when needed ('''For DNA extractions no sterilization required''') | ||
| + | [[Category:Media]] | ||
| + | [[Category:Stock_Solutions]] | ||
Revision as of 10:59, 20 January 2021
Lallzyme is a lytic enzyme that digests the cell wall of fungi. It is commercially available for wine production to digest botrytus.
It can also be used to digest fission yeast cells to generate protoplasts for example for DNA extraction
Even though it does not perform as well as zymolyase or [gluculase] per weight enzyme Flor-Parra et al 2014, doi:10.1002/yea.2994 it still performs much better per euro.
To use:
- Dissolve 50mg Lallzyme powder directly to 0.5 ml isotonic solution (e.g. Buffer Y1)
- Mix until dissolved
- Filter sterilize when needed (For DNA extractions no sterilization required)
