Difference between revisions of "Glycerol"
From PombEvolution
Jump to navigationJump to search (Created page with "== Glycerol or [https://www.youtube.com/watch?v=hOllF3TgAsM glycerin] == We mainly use glycerol as a "cryoprotectant" to store ''E. coli'', fungal mycelia or yeast cells in s...") |
|||
| Line 1: | Line 1: | ||
== Glycerol or [https://www.youtube.com/watch?v=hOllF3TgAsM glycerin] == | == Glycerol or [https://www.youtube.com/watch?v=hOllF3TgAsM glycerin] == | ||
| − | We mainly use glycerol as a "cryoprotectant" to store ''E. coli'', fungal mycelia or yeast cells in suspended animation at -80°C. The glycerol in the medium/water disrupts the crystal lattice formation of ice crystals, thereby reducing damage by ice crystals. | + | We mainly use glycerol as a "cryoprotectant" to [[Storage_of_Strains | store]] ''E. coli'', fungal mycelia or yeast cells in suspended animation at -80°C. The glycerol in the medium/water disrupts the crystal lattice formation of ice crystals, thereby reducing damage by ice crystals. |
Sterilize Glycerol by filtration (preferred) or autoclaving (what we actually do, because easier, but this produces some acrolein which is mutagenic and auto fluorescent). <br> | Sterilize Glycerol by filtration (preferred) or autoclaving (what we actually do, because easier, but this produces some acrolein which is mutagenic and auto fluorescent). <br> | ||
Revision as of 16:35, 4 October 2018
Glycerol or glycerin
We mainly use glycerol as a "cryoprotectant" to store E. coli, fungal mycelia or yeast cells in suspended animation at -80°C. The glycerol in the medium/water disrupts the crystal lattice formation of ice crystals, thereby reducing damage by ice crystals.
Sterilize Glycerol by filtration (preferred) or autoclaving (what we actually do, because easier, but this produces some acrolein which is mutagenic and auto fluorescent).
Make a dilution for 2X the appropriate concentration.
We use:
- 50% for E. coli --> final concentration 25%
- 30% for yeast and mycelia --> final concentration 15%
