Difference between revisions of "5-Fluoroorotic Acid (5-FOA)"
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* Keep plates in fridge. | * Keep plates in fridge. | ||
| − | For a good protocol on how to use this with YES, see: | + | For a good protocol on how to use this with YES, see: [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526333/ Petersen & Russell] |
[[Category:Media]] | [[Category:Media]] | ||
[[Category:Stock Solutions]] | [[Category:Stock Solutions]] | ||
Revision as of 14:26, 16 September 2019
5-FOA (C5H3FN2O) counter selects for ura+ strains. The nontoxic 5-FOA is converted to toxic 5-flurouracil in yeast with a functional ura4 gene, which normally synthesizes uracil. We can thus select for strains that are ura-.
- Make a 100X stock solution of 5-FOA in DMSO by adding 100 mg/ml.
- For use, use 1:100 (200 µl/plate).
- Stock solution is available in freezer 7.
- Keep plates in fridge.
For a good protocol on how to use this with YES, see: Petersen & Russell
