Difference between revisions of "YES - Yeast Extract Supplemented"
From PombEvolution
Jump to navigationJump to search (Created page with "== Yeast Extract Supplemented (YES or YEA with the A for Additives) == This is a rich medium used for optimal growth of cells. <BR> As the content of Yeast Extract and CAS can...") |
|||
| Line 26: | Line 26: | ||
<BR> | <BR> | ||
| − | Sterilize by autoclaving for 15min at | + | Sterilize by autoclaving for 15min at 121°C. Glucose can be added before autoclaving. |
[[Category:Media]] | [[Category:Media]] | ||
Revision as of 17:53, 1 February 2019
Yeast Extract Supplemented (YES or YEA with the A for Additives)
This is a rich medium used for optimal growth of cells.
As the content of Yeast Extract and CAS can differ between batches, growth can potentially be affected by ingredients and thus this type of ‘complex’ medium is not preferred for experimental evolution etc.
Note that YES contains thiamine and hence will repress the nmt1 promotor (e.g. used in the fluorescent strains). For these strains use EMM.
Supplements are available in stock solution in the cupboards near the S2 hood.
| Ingredient | Amount/ liter ddH2O |
|---|---|
| Yeast extract | 5 g |
| CAS amino acids | 2 g |
| D-Glucose | 30 g (50 ml of 60% Glucose solution) |
| Adenine | 100mg (10ml stock) |
| L-leucine | 225 mg (10ml stock) |
| Histidine | 225 mg (10ml stock) |
| Uracil | 225 mg (10ml stock) |
For solid medium add 20g/L agar(2%)
Sterilize by autoclaving for 15min at 121°C. Glucose can be added before autoclaving.
