Phleomycin

From PombEvolution
Revision as of 16:07, 9 October 2018 by Admin (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Antibiotic to select against yeast and filamentous fungi.

Selective antibiotic for the Sh ble gene


Make 5 ml of stock solution 20 mg/ml

  • Weigh 125 mg of Phleomycin.
  • Add 5 ml of H2O. Disolve completely.
  • Sterilize by drawing the solution through a syringe filter .

Usage

For Fission yeast and for Schizophyllum

Dilute 1:2000 to final concentration of 10 µl/ml (12µl for 9cm plate)