Difference between revisions of "Phleomycin"
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| − | Antibiotic to select against yeast and fungi | + | Antibiotic to select against yeast and filamentous fungi. |
Selective antibiotic for the ''Sh ble'' gene | Selective antibiotic for the ''Sh ble'' gene | ||
Latest revision as of 17:07, 9 October 2018
Antibiotic to select against yeast and filamentous fungi.
Selective antibiotic for the Sh ble gene
Make 5 ml of stock solution 20 mg/ml
- Weigh 125 mg of Phleomycin.
- Add 5 ml of H2O. Disolve completely.
- Sterilize by drawing the solution through a syringe filter .
Usage
For Fission yeast and for Schizophyllum
Dilute 1:2000 to final concentration of 10 µl/ml (12µl for 9cm plate)
