Difference between revisions of "PMG - Pombe Minimal Glutamate"

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(Added modification for using L-glutamic acid)
Line 12: Line 12:
 
|Na<sub>2</sub>HPO<sub>4</sub>.2H<sub>2</sub>O (or anhydrous)||2.76 g (2.2 g)
 
|Na<sub>2</sub>HPO<sub>4</sub>.2H<sub>2</sub>O (or anhydrous)||2.76 g (2.2 g)
 
|-
 
|-
|L-Glutamic acid monosodium salt hydrate (glutamate)                         ||    5.0 g  
+
|L-Glutamic acid monosodium salt hydrate  
 +
(or L-Glutamic acid)  
 +
| 5.0 g (3.75 g)
 
|-
 
|-
 
|D-Glucose                      || 20 g (=2% final concentration)
 
|D-Glucose                      || 20 g (=2% final concentration)
Line 22: Line 24:
 
|[[Mineral Stock x10.000]]          ||0.1 ml  
 
|[[Mineral Stock x10.000]]          ||0.1 ml  
 
|}
 
|}
 +
 +
When using L-Glutamic acid, set to pH 5.8 using NaOH 10N
  
 
Add supplements as required (225mg/L for most, 100mg/L for Adenine).<BR>  
 
Add supplements as required (225mg/L for most, 100mg/L for Adenine).<BR>  
 
For solid medium add 2% Agar-agar (20g/L)
 
For solid medium add 2% Agar-agar (20g/L)

Revision as of 13:56, 4 July 2022

Edinburgh Minimal Medium Glutamate (EMMG, PMG)

Modification of EMM in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on EMM (especially G418). On this medium strains do cross so the medium can also be used to test for mating types. However, it is much richer than the crossing mediums EMM-lowN or EMM-N, so mating takes more time (about 3 days).

Ingredient Amount per liter
Potassium Hydrogen Phthallate 3.0 g
Na2HPO4.2H2O (or anhydrous) 2.76 g (2.2 g)
L-Glutamic acid monosodium salt hydrate

(or L-Glutamic acid)

5.0 g (3.75 g)
D-Glucose 20 g (=2% final concentration)
Salts Stock x50 20 ml
Vitamin Stock x1000 1.0 ml
Mineral Stock x10.000 0.1 ml

When using L-Glutamic acid, set to pH 5.8 using NaOH 10N

Add supplements as required (225mg/L for most, 100mg/L for Adenine).
For solid medium add 2% Agar-agar (20g/L)