Difference between revisions of "PMG - Pombe Minimal Glutamate"
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== Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | == Edinburgh Minimal Medium Glutamate (EMMG, PMG) == | ||
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|L-Glutamic acid monosodium salt hydrate (glutamate) || 5.0 g | |L-Glutamic acid monosodium salt hydrate (glutamate) || 5.0 g | ||
|- | |- | ||
| − | |D-Glucose || | + | |D-Glucose || 20 g (=2% final concentration) |
|- | |- | ||
|[[Salts Stock x50]] ||20 ml | |[[Salts Stock x50]] ||20 ml | ||
Revision as of 16:23, 27 April 2021
Edinburgh Minimal Medium Glutamate (EMMG, PMG)
Modification of EMM in which Glutamate is used as a substitute for Ammonium chloride. This medium can be used with antibiotics that do not work on EMM (especially G418). On this medium strains do cross so the medium can also be used to test for mating types. However, it is much richer than the crossing mediums EMM-lowN or EMM-N, so mating takes more time (about 3 days).
| Ingredient | Amount per liter |
|---|---|
| Potassium Hydrogen Phthallate | 3.0 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) |
| L-Glutamic acid monosodium salt hydrate (glutamate) | 5.0 g |
| D-Glucose | 20 g (=2% final concentration) |
| Salts Stock x50 | 20 ml |
| Vitamin Stock x1000 | 1.0 ml |
| Mineral Stock x10.000 | 0.1 ml |
Add supplements as required (225mg/L for most, 100mg/L for Adenine).
For solid medium add 2% Agar-agar (20g/L)
