Modified Schizophyllum Minimal Medium

Glycerol Schizophyllum minimal media (GSMM)

Modified minimal medium for Schizophyllum commune in which glycerol substitutes glucose. From Ohm et al. 2010 NatBiotech modified from Dons et al. 1979

Dissolve in demi-water:

Ingredient	Per 1 liter	2X solution
Glycerol	40 ml	80 ml
KH2PO4	0.46 g	0.92 g
K2HPO4. 3 H2O	1.28 g	2.56 g
MgSO4. 7 H2O	0.5 g	1 g
Trace elements solution	1 ml	2 ml
FeCl3 solution	1 ml (=5mg)	2 ml
L-Asparagin	1.5 g	3 g
Agar	20 g (2%)	-

Add after Autoclaving:

1.0 ml of Filter sterilized 1000X Thiamine stock solution (12mg/100ml) (or 2 ml/L for 2X)