Glucose Stock Solution 60%
From PombEvolution
Jump to navigationJump to searchFor 800ml
- Weigh 480g D-Glucose
- Add to a 1 liter bottle
- Add 500ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 800ml
For 300ml
- Weigh 180g D-Glucose
- Add to a 500ml bottle
- Add 180ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 300ml
Sterilize
- Sterilize by filter sterilization (there are filters for large volumes in the storage room) OR
- by sterilizing by Autoclaving at 110C for 10 min (short program to avoid caramelization which can affect background fluorescence)