Difference between revisions of "Glucose Stock Solution 60%"
From PombEvolution
Jump to navigationJump to searchm |
|||
Line 17: | Line 17: | ||
== Sterilize == | == Sterilize == | ||
* Sterilize by [[Filter sterilize|filter sterilization]] (there are filters for large volumes in the storage room) '''OR''' | * Sterilize by [[Filter sterilize|filter sterilization]] (there are filters for large volumes in the storage room) '''OR''' | ||
− | * by sterilizing by [[Autoclaving]] at 110C for 10 min (short program to avoid caramelization which can affect | + | * by sterilizing by [[Autoclaving]] at 110C for 10 min (short program to avoid caramelization which can affect background fluorescence) |
[[Category:Stock Solutions]] | [[Category:Stock Solutions]] | ||
[[Category:Media]] | [[Category:Media]] |
Latest revision as of 09:29, 29 June 2022
For 800ml
- Weigh 480g D-Glucose
- Add to a 1 liter bottle
- Add 500ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 800ml
For 300ml
- Weigh 180g D-Glucose
- Add to a 500ml bottle
- Add 180ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 300ml
Sterilize
- Sterilize by filter sterilization (there are filters for large volumes in the storage room) OR
- by sterilizing by Autoclaving at 110C for 10 min (short program to avoid caramelization which can affect background fluorescence)