Difference between revisions of "Glucose Stock Solution 60%"

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== Sterilize ==
 
== Sterilize ==
 
* Sterilize by [[Filter sterilize|filter sterilization]] (there are filters for large volumes in the storage room) '''OR'''
 
* Sterilize by [[Filter sterilize|filter sterilization]] (there are filters for large volumes in the storage room) '''OR'''
* by sterilizing by [[Autoclaving]] at 110C for 10 min (short program to avoid caramelization which can affect back ground fluorescence)
+
* by sterilizing by [[Autoclaving]] at 110C for 10 min (short program to avoid caramelization which can affect background fluorescence)
  
 
[[Category:Stock Solutions]]
 
[[Category:Stock Solutions]]
 
[[Category:Media]]
 
[[Category:Media]]

Latest revision as of 09:29, 29 June 2022

For 800ml

  • Weigh 480g D-Glucose
  • Add to a 1 liter bottle
  • Add 500ml dd-water
  • Stick in microwave and quickly heat
  • Mix to dissolve all the sugar
  • Add more dd-Water to a final volume of 800ml

For 300ml

  • Weigh 180g D-Glucose
  • Add to a 500ml bottle
  • Add 180ml dd-water
  • Stick in microwave and quickly heat
  • Mix to dissolve all the sugar
  • Add more dd-Water to a final volume of 300ml

Sterilize

  • Sterilize by filter sterilization (there are filters for large volumes in the storage room) OR
  • by sterilizing by Autoclaving at 110C for 10 min (short program to avoid caramelization which can affect background fluorescence)