Difference between revisions of "Glucose Stock Solution 60%"
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Revision as of 10:56, 20 January 2021
For 800ml
- Weigh 480g D-Glucose
- Add to a 1 liter bottle
- Add 500ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 800ml
For 300ml
- Weigh 180g D-Glucose
- Add to a 500ml bottle
- Add 180ml dd-water
- Stick in microwave and quickly heat
- Mix to dissolve all the sugar
- Add more dd-Water to a final volume of 300ml
Sterilize
- Sterilize by filter sterilization (there are filters for large volumes in the storage room) OR
- by sterilizing by Autoclaving at 110C for 10 min (short program to avoid caramelization which can affect back ground fluorescence)