ExoSap PCR cleanup

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Before performing the sequencing reaction using the Big Dye Setup, cleanup your PCR using this protocol.

Take your PCR tube with sample:

  • add 1.5μl Exo-SAP enzyme mix
  • incubate in a PCR machine using program named 'exo'
    1. 37°C for 30 min (remove primers, dNTPs, etc.)
    2. 80°C for 15 min (inactivate enzymes)
    3. 7°C for infinite