EMM - Edinburgh Minimal Medium

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Edinburgh Minimal Medium (EMM, EMM2, or EMM Phthallate)

This is a synthetic defined minimal medium EMM2 is used for vegetative growth.
The medium is also used to screen strains for deficiencies and prototrophic markers.
We use this medium during the asexual phase of all our experimental evolution.
Furthermore, Edinburgh Minimal Media is a thiamine-free medium that does not suppress the nmt1 promoter.
The medium can be modified to a Mating medium by leaving out ammoniumchloride as a nitrogen source (EMM-N) or modified to PMG.

Ingredient Amount per liter Amount per 800ml bottle
Potassium Hydrogen Phthallate 3.0 g 2.4 g
Na2HPO4.2H2O (or anhydrous) 2.76 g (2.2 g) 2.2 g (1.76 g)
NH4Cl 5.0 g 4 g
Salts Stock x50 20 ml 16 ml
Vitamin Stock x1000 1.0 ml 0.8 ml
Mineral Stock x10.000 0.1 ml 80 µl
Glucose 20 g 16 g

Sterilize by autoclaving for 15min at 121C.


Add 33.3 ml (26.6 ml per bottle) sterile 60% Glucose per liter after autoclaving the medium (if added before, this interacts with ammonium chloride resulting in almost black medium).

Add supplements as required (225mg/L for most, 100mg/L for Adenine) before autoclaving.

For solid medium add 2% Agar-agar (20g/L)