Difference between revisions of "EMM - Edinburgh Minimal Medium"
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Sterilize by autoclaving for 15min at 121C. | Sterilize by autoclaving for 15min at 121C. | ||
| − | <BR>Add 33.3 ml (26.6 ml per bottle) sterile [[Glucose Stock Solution 60%|60% Glucose]] per liter after autoclaving the medium (if added before, this interacts with ammonium chloride resulting in almost black medium).<BR> | + | <BR>Add 33.3 ml (26.6 ml per bottle) sterile [[Glucose Stock Solution 60%|60% Glucose]] per liter after autoclaving the medium (if added before, this interacts with [[Ammonium Chloride|ammonium chloride]] resulting in almost black medium).<BR> |
Add supplements as required (225mg/L for most, 100mg/L for Adenine) before autoclaving.<BR> | Add supplements as required (225mg/L for most, 100mg/L for Adenine) before autoclaving.<BR> | ||
For solid medium add 2% Agar-agar (20g/L) | For solid medium add 2% Agar-agar (20g/L) | ||
Latest revision as of 13:19, 24 November 2022
Edinburgh Minimal Medium (EMM, EMM2, or EMM Phthallate)
This is a synthetic defined minimal medium EMM2 is used for vegetative growth.
The medium is also used to screen strains for deficiencies and prototrophic markers.
We use this medium during the asexual phase of all our experimental evolution.
Furthermore, Edinburgh Minimal Media is a thiamine-free medium that does not suppress the nmt1 promoter.
The medium can be modified to a Mating medium by leaving out ammoniumchloride as a nitrogen source (EMM-N) or modified to PMG.
| Ingredient | Amount per liter | Amount per 800ml bottle |
|---|---|---|
| Potassium Hydrogen Phthallate | 3.0 g | 2.4 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) | 2.2 g (1.76 g) |
| NH4Cl | 5.0 g | 4 g |
| Salts Stock x50 | 20 ml | 16 ml |
| Vitamin Stock x1000 | 1.0 ml | 0.8 ml |
| Mineral Stock x10.000 | 0.1 ml | 80 µl |
| Glucose | 20 g | 16 g |
Sterilize by autoclaving for 15min at 121C.
Add 33.3 ml (26.6 ml per bottle) sterile 60% Glucose per liter after autoclaving the medium (if added before, this interacts with ammonium chloride resulting in almost black medium).
Add supplements as required (225mg/L for most, 100mg/L for Adenine) before autoclaving.
For solid medium add 2% Agar-agar (20g/L)
