EMM-lowN
From PombEvolution
Jump to navigationJump to searchEdinburgh Minimal Medium - low Nitrogen (EMM-lowN)
Modification of EMM used for crosses and to test mating types, but richer than the other crossing medium EMM-N which allows for some growth and thus more asci. Like in most fungi, low nitrogen induces mating in pombe too.
Note that this is different from PMG which has much more Glutamic acid.
| Ingredient | Amount per liter |
|---|---|
| Potassium Hydrogen Phthallate | 3.0 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) |
| L-Glutamic acid monosodium salt hydrate (glutamate) | 1.0 g |
| D-Glucose | 20 g (2%) |
| Salts Stock x50 | 20 ml |
| Vitamin Stock x1000 | 1.0 ml |
| Mineral Stock x10.000 | 0.1 ml |
| Uracil | 200 mg |
| Adenine | 80 mg |
| Leucine | 200 mg |
| Histidine | 200 mg |
For solid medium add 2% Agar-agar (20g/L) separately to each bottle (only dissolves during autoclaving).
