Difference between revisions of "EMM-N - Minimal Mating Medium"
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The medium is also used to create [[Synchronized_%27Competent%27_Yeast_Cells | chemically competent cells for transformations]].<BR> | The medium is also used to create [[Synchronized_%27Competent%27_Yeast_Cells | chemically competent cells for transformations]].<BR> | ||
It is identical to [[EMM_-_Edinburgh_Minimal_Medium|EMM]], except for the omission of NH<sub>4</sub>Cl.<br> | It is identical to [[EMM_-_Edinburgh_Minimal_Medium|EMM]], except for the omission of NH<sub>4</sub>Cl.<br> | ||
| − | Note that due to complete lack of a nitrogen source this medium does not support growth. For most crosses it's better to use [[ | + | Note that due to complete lack of a nitrogen source this medium does not support growth. For most crosses it's better to use [[EMM-lowN | mating medium]] |
{| class="wikitable" | {| class="wikitable" | ||
Revision as of 13:50, 19 March 2020
Edinburgh Minimal Medium without Nitrogen (EMM-N)
This is a synthetic defined minimal medium used to induce mating or induce mating competence.
The medium is also used to create chemically competent cells for transformations.
It is identical to EMM, except for the omission of NH4Cl.
Note that due to complete lack of a nitrogen source this medium does not support growth. For most crosses it's better to use mating medium
| Ingredient | Amount per liter |
|---|---|
| Potassium Hydrogen Phthallate | 3.0 g |
| Na2HPO4.2H2O (or anhydrous) | 2.76 g (2.2 g) |
| D-Glucose | 20 g |
| Salts Stock x50 | 20 ml |
| Vitamin Stock x1000 | 1.0 ml |
| Mineral Stock x10.000 | 0.1 ml |
Add supplements as required (225mg/L for most, 100mg/L for Adenine).
However, be aware that adding too many supplements might affect mating efficiency as these supp can act as N-source.
For solid medium add 2% Agar-agar (20g/L)
