Buffer QF

From PombEvolution
Jump to navigationJump to search

Buffer QF (elution buffer) used for the QIAGEN Genomic-Tip DNA extraction protocol.

The buffer is used to elute the DNA from the column.

Buffer QF

  • 1.25 M NaCl
  • 50 mM Tris·Cl, pH 8.5
  • 15% isopropanol

Buffer QF can be stored at RT.

To make QF:

  • Dissolve in 200 ml distilled water:
  • 18.26 g NaCl
  • 1.52 g Tris base
  • Adjust the pH to 8.5 with HCl
  • Add 37.5 ml pure isopropanol
  • Adjust the volume to 250 ml with distilled water.