YE - Yeast Extract
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Jump to navigationJump to searchYeast Extract (YE )
This is a rich medium that can be used to check for mutations in the adenine pathway, specifically to verify the two complementing ade6 alleles ade6-M210 and ade6-M216.
As the content of Yeast Extract and CAS can differ between batches, growth can potentially be affected by ingredients and thus this type of ‘complex’ medium is not preferred for experimental evolution etc.
Note that YE contains thiamine and hence will repress the nmt1 promotor (e.g. used in the fluorescent strains). For these strains use EMM or PMG.
| Ingredient | Amount/ liter ddH2O |
|---|---|
| Yeast extract | 5 g |
| CAS amino acids | 2 g |
| D-Glucose | 30 g (50 ml of 60% Glucose solution) |
| L-leucine | 225 mg (10ml stock) |
| Histidine | 225 mg (10ml stock) |
| Uracil | 225 mg (10ml stock) |
For solid medium add 20g/L agar(2%)
Sterilize by autoclaving for 15min at 121C. Glucose can be added before autoclaving.
