YES - Yeast Extract Supplemented
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Jump to navigationJump to searchYeast Extract Supplemented (YES or YEA with the A for Additives)
This is a rich medium used for optimal growth of cells.
As the content of Yeast Extract and CAS can differ between batches, growth can potentially be affected by ingredients and thus this type of ‘complex’ medium is not preferred for experimental evolution etc.
Note that YES contains thiamine and hence will repress the nmt1 promotor (e.g. used in the fluorescent strains). For these strains use EMM.
Supplements are available in stock solution in the cupboards near the S2 hood.
| Ingredient | Amount/ liter ddH2O | Amount/ per 800ml |
|---|---|---|
| Yeast extract | 5 g | 4 g |
| CAS amino acids | 2 g | 1.6 g |
| D-Glucose | 30 g (50 ml of 60% Glucose solution) | 24 g (40ml) |
| Adenine | 100 mg (10ml stock) | 80 mg |
| L-leucine | 225 mg (10ml stock) | 180 mg |
| Histidine | 225 mg (10ml stock) | 180 mg |
| Uracil | 225 mg (10ml stock) | 180 mg |
For solid medium add 20g/L agar(2%)
Supplements premixed: add 775 mg (or 620 mg per 800ml)
Sterilize by autoclaving for 15min at 121°C. Glucose can be added before autoclaving.
