Miniprep

Minipreps are methods to extract plasmid DNA molecules from E. coli or Yeast cells.

In our lab we use a variety of methods.

1. Easiest is to use a Miniprep kit from Macherey-Nagel that uses columns on overnight cultures of E. coli. This method yields clean and rather high yields of plasmid DNA
2. When many samples need to be tested for integrity, a cheap and dirty miniprep method can be used.
3. Finally, the first method can be modified to return higher yields, however at the cost of DNA integrity.